Degradation of luteinizing hormone-releasing hormone (LHRH) and an LHRH agonist by the rat testis.

@article{Hedger1986DegradationOL,
  title={Degradation of luteinizing hormone-releasing hormone (LHRH) and an LHRH agonist by the rat testis.},
  author={Mark P Hedger and David M. Robertson and S J Tepe and Cheryl A. Browne and David Morritz de Kretser},
  journal={Molecular and cellular endocrinology},
  year={1986},
  volume={46 1},
  pages={59-70}
}
Degradation of LHRH and [D-Ser(tBu)6,des-Gly-NH10(2)]LHRH ethylamide (LHRH-A), during incubation with high-speed supernatants of rat testes, as assessed by reversed-phase (RP)-HPLC fractionation of the iodinated peptides and by radioimmunoassays for LHRH or LHRH-A, was principally due to a neutral 43 000 Da peptidase with apparent Km values at 25 degrees C of 0.15 microM for LHRH and 1.19 microM for LHRH-A. The peptidase was inhibited by sulphydryl reagents, TLCK, 1,10-phenanthroline, EDTA… CONTINUE READING