Degradation of intact basement membranes by human and murine tumor enzymes.

@article{Karakiulakis1988DegradationOI,
  title={Degradation of intact basement membranes by human and murine tumor enzymes.},
  author={George Karakiulakis and Eleftheria Missirlis and Anthony H. Aletras and Michael E. Maragoudakis},
  journal={Biochimica et biophysica acta},
  year={1988},
  volume={967 2},
  pages={163-75}
}
Homogenates from malignant tumors, obtained from surgery specimens or from transplants of Walker 256 carcinosarcoma in rats, contained an enzyme activity capable of degrading intact 3H-acetylated basement membranes from bovine lens. The enzyme activity from murine tumor was purified about 7500-fold by (NH4)2SO4 fractionation, ion exchange and gel chromatography. The apparent molecular weight of the purified enzyme was approximately 50,000. The rate of degradation of 3H-labelled basement… CONTINUE READING