Degradation of hammerhead ribozymes by human ribonucleases

  title={Degradation of hammerhead ribozymes by human ribonucleases},
  author={Lu Gui Qiu and Alexandra M{\^o}nego Moreira and G. Kaplan and Ruth Levitz and J. Wang and Changhong Xu and Karl Drlica},
  journal={Molecular and General Genetics MGG},
Hammerhead ribozymes were used as substrates to examine endoribonucleolytic activities in cell extracts and cultured human cells. Primer-extension analyses showed that ribozymes directed against tumor necrosis factor-α mRNA and human immunodeficiency virus type 1 tat mRNA were cleaved at UA and CA dinucleotides by extracts. Preferred cleavage sites were similar to those observed following digestion with RNase A, and cleavage was blocked by RNasin, an inhibitor of pyrimidine-specific… CONTINUE READING
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