• DOI:10.1271/BBB1961.44.1773
• Corpus ID: 87550178

Degradation of Arginine via α-Keto-δ-Guanidinovalerate and α-Keto-δ-Aminovalerate in Arginine-Grown Arthrobacter simplex

@article{Tochikura1980DegradationOA,
  title={Degradation of Arginine via $\alpha$-Keto-$\delta$-Guanidinovalerate and $\alpha$-Keto-$\delta$-Aminovalerate in Arginine-Grown Arthrobacter simplex},
  author={Tatsurokuro Tochikura and K Sugiyama and Masayasu Bunno and T Matsubara and Takashi Tachiki},
  journal={Agricultural and biological chemistry},
  year={1980},
  volume={44},
  pages={1773-1778}
}

• T. Tochikura, K. Sugiyama, +2 authors T. Tachiki
• Published 1 August 1980
• Chemistry, Biology
• Agricultural and biological chemistry

Arthrobacter simplex was screened as an α-keto-δ-guanidinovalerate (ketoarginine) assimilating organism. A characteristic feature was its growth on ketoarginine as a carbon source; it began to grow after an extremely long lag. Its growth was stimulated by addition of 0.02% yeast extract to the medium.The results indicated the transamination of arginine-α-ketoglutarate (α-KGA) and the hydrolyzing reaction of ketoarginine into α-keto-δ-aminovalerate and urea. Two intermediates, ketoarginine and α-keto-δ-aminovalerate, were isolated and identified by various procedures. Coupling of the two reactions was demonstrated in cell-free extracts of arginine-grown cells; ketoarginine formed from arginine by transamination with α-KGA was hydrolyzed directly to α-keto-δ-aminovalerate and urea. The metabolic routes of arginine in microorganisms were discussed.