Degradation Parameters from Pulse-Chase Experiments

  title={Degradation Parameters from Pulse-Chase Experiments},
  author={Celine Sin and Davide Chiarugi and Angelo Valleriani},
  booktitle={PloS one},
Pulse-chase experiments are often used to study the degradation of macromolecules such as proteins or mRNA. Considerations for the choice of pulse length include the toxicity of the pulse to the cell and maximization of labeling. In the general case of non-exponential decay, varying the length of the pulse results in decay patterns that look different. Analysis of these patterns without consideration to pulse length would yield incorrect degradation parameters. Here we propose a method that… CONTINUE READING

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