Deglycosylation of cellulosomal enzyme enhances cellulosome assembly in Saccharomyces cerevisiae.

@article{Suzuki2012DeglycosylationOC,
  title={Deglycosylation of cellulosomal enzyme enhances cellulosome assembly in Saccharomyces cerevisiae.},
  author={Hiroaki Suzuki and Takao Imaeda and Takao Kitagawa and Katsunori Kohda},
  journal={Journal of biotechnology},
  year={2012},
  volume={157 1},
  pages={64-70}
}
We have estimated the effects of hyper-mannosylation of dockerin-type cellulase on cellulosome assembly by using Saccharomyces cerevisiae and 44 protein glycosylation mutants, because the heterologous protein displayed on yeast is assumed to be modified by yeast-specific hyper-mannosylation. First, we constructed the yeast strain CtminiCipA, which displays a heterologous scaffolding protein (miniCipA from Clostridium thermocellum) on its cell surface, and glycosylation mutants secreting a… CONTINUE READING

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