Degeneration in vivo of rat hippocampal neurons by wild-type Alzheimer amyloid precursor protein overexpressed by adenovirus-mediated gene transfer.

@article{Nishimura1998DegenerationIV,
  title={Degeneration in vivo of rat hippocampal neurons by wild-type Alzheimer amyloid precursor protein overexpressed by adenovirus-mediated gene transfer.},
  author={Isao Nishimura and Taichi Uetsuki and Samir Dani and Yoshiyuki Ohsawa and Izumu Saito and Hitoshi Okamura and Yasuo Uchiyama and Kazuaki Yoshikawa},
  journal={The Journal of neuroscience : the official journal of the Society for Neuroscience},
  year={1998},
  volume={18 7},
  pages={2387-98}
}
In an attempt to elucidate the pathological implications of intracellular accumulation of the amyloid precursor protein (APP) in postmitotic neurons in vivo, we transferred APP695 cDNA into rat hippocampal neurons by using a replication-defective adenovirus vector. We first improved the efficiency of adenovirus-mediated gene transfer into neurons in vivo by using hypertonic mannitol. When a beta-galactosidase-expressing recombinant adenovirus suspended in 1 M mannitol was injected into a dorsal… CONTINUE READING