Degenerate primer design for highly variable genomes.

Abstract

The application of degenerate PCR primers towards target amplification and sequencing is a useful technique when a population of organisms under investigation is evolving rapidly, or is highly diverse. Degenerate bases in these primers are specified with ambiguity codes that represent alternative nucleotide configurations. Degenerate PCR primers allow the… (More)
DOI: 10.1007/978-1-4939-2365-6_7

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