Degenerate oligonucleotide primed-polymerase chain reaction-based array comparative genomic hybridization for extensive amplicon profiling of breast cancers : a new approach for the molecular analysis of paraffin-embedded cancer tissue.

@article{Daigo2001DegenerateOP,
  title={Degenerate oligonucleotide primed-polymerase chain reaction-based array comparative genomic hybridization for extensive amplicon profiling of breast cancers : a new approach for the molecular analysis of paraffin-embedded cancer tissue.},
  author={Yataro Daigo and Soo F Chin and Kylie L. Gorringe and Lynda G. Bobrow and Bruce A. J. Ponder and Paul D P Pharoah and Carlos Caldas},
  journal={The American journal of pathology},
  year={2001},
  volume={158 5},
  pages={1623-31}
}
We have developed a protocol for degenerate oligonucleotide-primed-polymerase chain reaction-based array comparative genomic hybridization (array CGH) that, when combined with a laser microdissection technique, allows the analysis of cancer cell populations isolated from routine, formalin-fixed, paraffin-embedded tissue samples. Comparison of copy number changes detected by degenerate oligonucleotide-primed-polymerase chain reaction-based array CGH to those detected by conventional array CGH or… CONTINUE READING