Degenerate oligonucleotide-primed PCR (DOP-PCR): evaluation of its reliability for screening of genetic alterations in neoplasia.

@article{SnchezCspedes1998DegenerateOP,
  title={Degenerate oligonucleotide-primed PCR (DOP-PCR): evaluation of its reliability for screening of genetic alterations in neoplasia.},
  author={Montserrat S{\'a}nchez-C{\'e}spedes and Paul M. Cairns and Jin Jen and David Sidransky},
  journal={BioTechniques},
  year={1998},
  volume={25 6},
  pages={
          1036-8
        }
}
Genetic alterations underlie the development and progression of neoplasia. Screening for genetic alterations in biopsies and premalignant lesions, where the neoplastic tissue analyzed usually constitutes only a small fraction of the cells in the specimen, often is difficult and tedious. Even after successful isolation of DNA, limited quantities allow only a few genetic analyses, limiting the identification of genetic changes in small lesions and the establishment of molecular cancer progression… CONTINUE READING

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