Degenerate oligonucleotide gene shuffling (DOGS): a method for enhancing the frequency of recombination with family shuffling.

@article{Gibbs2001DegenerateOG,
  title={Degenerate oligonucleotide gene shuffling (DOGS): a method for enhancing the frequency of recombination with family shuffling.},
  author={Moreland David Gibbs and Kaisu Milja Helena Nevalainen and Peter L. Bergquist},
  journal={Gene},
  year={2001},
  volume={271 1},
  pages={
          13-20
        }
}
Improvement of the biochemical characteristics of enzymes has been aided by misincorporation mutagenesis and DNA shuffling. Shuffling techniques can be used on a collection of mutants of the same gene, or related families of genes can be shuffled to produce mutants encoding chimeric gene products. One difficulty with current shuffling procedures is the predominance of unshuffled ("parental") molecules in the pool of mutants. We describe a procedure for gene shuffling using degenerate primers… CONTINUE READING
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