Defoliation induces fructan 1-exohydrolase II in Witloof chicory roots. Cloning and purification of two isoforms, fructan 1-exohydrolase IIa and fructan 1-exohydrolase IIb. Mass fingerprint of the fructan 1-exohydrolase II enzymes.

@article{Ende2001DefoliationIF,
  title={Defoliation induces fructan 1-exohydrolase II in Witloof chicory roots. Cloning and purification of two isoforms, fructan 1-exohydrolase IIa and fructan 1-exohydrolase IIb. Mass fingerprint of the fructan 1-exohydrolase II enzymes.},
  author={Wim Van den Ende and An Michiels and D Van Wonterghem and Stefan Clerens and Joke De Roover and Andr{\'e} van Laere},
  journal={Plant physiology},
  year={2001},
  volume={126 3},
  pages={1186-95}
}
The cloning of two highly homologous chicory (Cichorium intybus var. foliosum cv Flash) fructan 1-exohydrolase cDNAs (1-FEH IIa and 1-FEH IIb) is described. Both isoenzymes could be purified from forced chicory roots as well as from the etiolated "Belgian endive" leaves where the 1-FEH IIa isoform is present in higher concentrations. Full-length cDNAs were obtained by a combination of reverse transcriptase-polymerase chain reaction (PCR), PCR and 5'- and 3'-rapid amplification of cDNA ends… CONTINUE READING

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References

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Showing 1-10 of 44 references

Characterization of two members of the maize

  • Kim J-Y, A Mahé, +4 authors Prioul J-L
  • GENE
  • 2000

In vitro synthesis of fructofuranosyl - only oligosaccharides from inulin and fructose by purified chicory root fructan : fructan fructosyl transferase

  • W VandenEnde, J Roover, A Laere
  • Physiol Plant
  • 1999

Properties of fructosyltransferases involved in the synthesis of fructan in Liliaceous plants

  • A Sturm
  • J Plant Physiol
  • 1999
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