Defining the chemical groups essential for Tetrahymena group I intron function by nucleotide analog interference mapping.

@article{Strobel1997DefiningTC,
  title={Defining the chemical groups essential for Tetrahymena group I intron function by nucleotide analog interference mapping.},
  author={Scott A Strobel and Kirti Shetty},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={1997},
  volume={94 7},
  pages={2903-8}
}
Improved atomic resolution biochemical methods are needed to identify the chemical groups within an RNA that are essential to its activity. As a step toward this goal, we report the use of 5'-O-(1-thio)inosine monophosphate (IMP alphaS) in a nucleotide analog interference mapping (NAIM) assay that makes it possible to simultaneously, yet individually, determine the contribution of almost every N2 exocyclic amine of G within a large RNA. Using IMP alphaS, we identified the exocyclic amines that… CONTINUE READING
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