Defining the Transcriptome Assembly and Its Use for Genome Dynamics and Transcriptome Profiling Studies in Pigeonpea (Cajanus cajan L.)

@inproceedings{Dubey2011DefiningTT,
  title={Defining the Transcriptome Assembly and Its Use for Genome Dynamics and Transcriptome Profiling Studies in Pigeonpea (Cajanus cajan L.)},
  author={Anuja Dubey and Andrew D. Farmer and Jessica A. Schlueter and Steven B. Cannon and Brian L Abernathy and Reetu Tuteja and Jimmy Woodward and Trushar M. Shah and Benjamin Mulasmanovic and Himabindu Kudapa and Nikku L. Raju and Ragini Gothalwal and Suresh Pande and Yongli Xiao and Christopher D. Town and Nagendra Kumar Singh and Gregory D. May and Scott R. Jackson and Rajeev K. Varshney},
  booktitle={DNA research : an international journal for rapid publication of reports on genes and genomes},
  year={2011}
}
This study reports generation of large-scale genomic resources for pigeonpea, a so-called 'orphan crop species' of the semi-arid tropic regions. FLX/454 sequencing carried out on a normalized cDNA pool prepared from 31 tissues produced 494 353 short transcript reads (STRs). Cluster analysis of these STRs, together with 10 817 Sanger ESTs, resulted in a pigeonpea trancriptome assembly (CcTA) comprising of 127 754 tentative unique sequences (TUSs). Functional analysis of these TUSs highlights… CONTINUE READING