Defining in vivo dendritic cell functions using CD11c-DTR transgenic mice.

@article{BarOn2010DefiningIV,
  title={Defining in vivo dendritic cell functions using CD11c-DTR transgenic mice.},
  author={Liat Bar-On and Steffen Jung},
  journal={Methods in molecular biology},
  year={2010},
  volume={595},
  pages={429-42}
}
The study of dendritic cell involvement in complex phenomena that rely on multi-cellular interactions, such as immune homeostasis, stimulation, and tolerization, called for the investigation of dendritic cell functions within physiological context. To this end we have developed a conditional cell ablation strategy that is based on dendritic cell-restricted expression of a Diphtheria Toxin receptor (DTR) using the CD11c/Itgax promoter. Here, we provide basic protocols that describe the use of… CONTINUE READING