Defining desmosomal plakophilin-3 interactions

@article{Bonne2003DefiningDP,
  title={Defining desmosomal plakophilin-3 interactions},
  author={Stefan Bonné and Barbara Gilbert and Mechthild Hatzfeld and Xinyu Chen and Kathleen Janee Green and Frans van Roy},
  journal={The Journal of Cell Biology},
  year={2003},
  volume={161},
  pages={403 - 416}
}
Plakophilin 3 (PKP3) is a recently described armadillo protein of the desmosomal plaque, which is synthesized in simple and stratified epithelia. We investigated the localization pattern of endogenous and exogenous PKP3 and fragments thereof. The desmosomal binding properties of PKP3 were determined using yeast two-hybrid, coimmunoprecipitation and colocalization experiments. To this end, novel mouse anti-PKP3 mAbs were generated. We found that PKP3 binds all three desmogleins, desmocollin (Dsc… Expand
Plakophilin 1 but not plakophilin 3 regulates desmoglein clustering
TLDR
Dsg3 overexpression reconstituted cluster formation in Pkp3- but not Pk p1-deficient keratinocytes as shown by AFM and STED experiments and Extracellular crosslinking and AFM cluster mapping demonstrate that both Pkkp1 and 3 regulate Dsg membrane availability, whereas PkP1 but notPkP3 is required for Dsg3 clustering. Expand
The Desmosomal Plaque Proteins of the Plakophilin Family
TLDR
This paper gives an overview of the current knowledge on the very distinct roles of plakophilins in the cell and shows that junctional adhesion is not the only function of PKPs. Expand
Plakophilins: Multifunctional proteins or just regulators of desmosomal adhesion?
  • M. Hatzfeld
  • Biology, Medicine
  • Biochimica et biophysica acta
  • 2007
TLDR
This review aims to summarize the current knowledge of plakophilin function in the context of cell adhesion, signaling and their putative role in diseases. Expand
E-cadherin and plakoglobin recruit plakophilin3 to the cell border to initiate desmosome assembly
TLDR
The results reported here are consistent with the model that PG and E-cadherin recruit PKP3 to the cell border to initiate desmosome formation. Expand
Plakophilin-1 localizes to the nucleus and interacts with single-stranded DNA.
TLDR
In vitro assays suggest that in addition to mediating desmosome assembly, the nuclear pool of Pkp can influence cell survival by interactions with DNA. Expand
Palmitoylation of plakophilin is required for desmosome assembly
TLDR
It is demonstrated that multiple desmosomal components are palmitoylated in vivo, and plakophilin mutants unable to become palMIToylated act in a dominant-negative manner to disrupt proper localization of endogenous desmosome components and decrease Desmosomal adhesion. Expand
Plakophilins--hard work in the desmosome, recreation in the nucleus?
TLDR
The current knowledge concerning structure, function and regulation of the plakophilins that has been achieved during the last decade is summarized. Expand
Plakophilins 1 and 3 Bind to FXR1 and Thereby Influence the mRNA Stability of Desmosomal Proteins
TLDR
It is demonstrated that cytoplasmic PKP1/3 are components of mRNA ribonucleoprotein particles and act as posttranscriptional regulators of gene expression. Expand
Antagonistic Regulation of Intercellular Cohesion by Plakophilins 1 and 3.
TLDR
It is demonstrated that the balance between plakophilins 1 and 3 determines the context-dependent properties of epidermal desmosomes, which provides stable intercellular cohesion that resists mechanical stress, whereas plakophile 3 confers dynamics as required during tissue homeostasis and repair. Expand
Plakophilin 1: an important stabilizer of desmosomes
  • A. South
  • Biology, Medicine
  • Clinical and experimental dermatology
  • 2004
TLDR
In vitro data implicate plakophilin 1 in regulating desmosome integrity in response to low calcium concentrations and in altering the migratory properties of wounded keratinocyte sheets in culture, which further underscore the significant role of PKP1 in keratinocytes cell biology. Expand
...
1
2
3
4
5
...

References

SHOWING 1-10 OF 57 REFERENCES
Interaction of plakophilins with desmoplakin and intermediate filament proteins: an in vitro analysis.
TLDR
It is postulate that effective cellular regulatory mechanisms exist that prevent plakophilins from unscheduled IF-binding, and specific desmoplakin interactions with either PKP1, PKP2 or PKP3, or combinations thereof, are involved in the selective recruitment of plakphilins to the desmosomal plaques. Expand
Defining the Interactions Between Intermediate Filaments and Desmosomes
TLDR
A model whereby at desmosome sites within dividing epidermal cells, DP and PG anchor to desmosomal cadherins and to each other, forming an ordered array of nontransmembrane proteins that then bind to keratin IFs is suggested. Expand
Protein Binding and Functional Characterization of Plakophilin 2
TLDR
It is demonstrated that plakophilin 2 can interact directly with several desmosomal components, including desmoplakin, plakoglobin, desmoglein 1 and 2, and desmocollin 1a and 2a, and is able to associate with β-catenin through its head domain, and the expression of plakophon 2 in SW480 cells up-regulates the endogenous β- catenin/T cell factor-signaling activity. Expand
The Function of Plakophilin 1 in Desmosome Assembly and Actin Filament Organization
TLDR
The results demonstrate that plakophilin 1 contains two functionally distinct domains: the head domain, which could play a role in organizing the desmosomal plaque in suprabasal cells, and the armadillo repeatdomain, which might be involved in regulating the dynamics of the actin cytoskeleton. Expand
Plakophilin 3--a novel cell-type-specific desmosomal plaque protein.
TLDR
Using recombinant DNA and immunological techniques, a novel desmosomal plaque protein is identified that is closely related to plakophilins 1 and 2, both members of the "armadillo-repeat" multigene family, and named plakphilin 3 (PKP3). Expand
Desmosomal plakophilin 2 as a differentiation marker in normal and malignant tissues.
Plakophilin 2 (PKP2) is a widespread protein which shows a remarkable dual location: On the one hand, it appears as a constitutive karyoplasmic protein and on the other it is a desmosomal plaqueExpand
Molecular map of the desmosomal plaque.
TLDR
Several predictions from previous work were borne out by this study, but in other cases the observations yielded unexpected results, which have significant implications relating to molecular interactions in desmosomes and emphasise the importance of applying multiple and complementary approaches to biological investigations. Expand
Plakophilin 1 interferes with plakoglobin binding to desmoplakin, yet together with plakoglobin promotes clustering of desmosomal plaque complexes at cell-cell borders.
TLDR
The results suggest that more than one armadillo family member is required for normal assembly and clustering of the desmosomal plaque in the upper layers of the epidermis. Expand
The Head Domain of Plakophilin-1 Binds to Desmoplakin and Enhances Its Recruitment to Desmosomes
TLDR
This model suggests that epidermal lesions in patients lacking plakophilin-1 are a consequence of the loss of integrity resulting from a decrease in binding sites for desmoplakin and intermediate filaments at desmosomes. Expand
Targeted mutation of plakoglobin in mice reveals essential functions of desmosomes in the embryonic heart
TLDR
Plakoglobin is an essential component of myocardiac desmosomes and seems to play a crucial role in the sorting out of desmosomal and adherens junction components, and consequently in the architecture of intercalated discs and the stabilization of heart tissue. Expand
...
1
2
3
4
5
...