Defining conditions for microbroth antifungal susceptibility tests: influence of RPMI and RPMI-2% glucose on the selection of endpoint criteria.

@article{RodrguezTudela1995DefiningCF,
  title={Defining conditions for microbroth antifungal susceptibility tests: influence of RPMI and RPMI-2% glucose on the selection of endpoint criteria.},
  author={Juan Lu{\'i}s Rodr{\'i}guez-Tudela and Joaqu{\'i}n V Mart{\'i}nez-Su{\'a}rez},
  journal={The Journal of antimicrobial chemotherapy},
  year={1995},
  volume={35 6},
  pages={
          739-49
        }
}
We have compared amphotericin B, flucytosine, ketoconazole and fluconazole susceptibilities of 40 clinical isolates of Candida albicans by broth microdilution in two different media: RPMI 1640 (RPMI) and the same medium supplemented with 18 g of glucose per L (RPMI-2% glucose). Preparation of media, drugs and inocula, as well as incubation temperature, followed the recommendations of the National Committee for Clinical Laboratory Standards (Villanova, PA, USA) antifungal agent working group for… CONTINUE READING
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