Defined large-scale alterations of the human cytomegalovirus genome constructed by cotransfection of overlapping cosmids.

@article{Kemble1996DefinedLA,
  title={Defined large-scale alterations of the human cytomegalovirus genome constructed by cotransfection of overlapping cosmids.},
  author={George W. Kemble and Gregory M. Duke and Ralf Winter and Richard R Spaete},
  journal={Journal of virology},
  year={1996},
  volume={70 3},
  pages={2044-8}
}
We have constructed defined human cytomegalovirus (CMV) mutants by cotransfecting overlapping cosmid clones spanning the 230-kbp genome. Using this strategy, we have introduced a 13-kbp region of DNA from a virulent strain of CMV into a defined position within the avirulent CMV(Towne) genome. Although more than 80% of the genome of these recombinant viruses was derived from Towne DNA, their plaque morphology more closely resembled that of Toledo. To date, CMV is the largest virus and requires… CONTINUE READING