Defined Medium Conditions for the Induction and Expansion of Human Pluripotent Stem Cell-Derived Retinal Pigment Epithelium

  title={Defined Medium Conditions for the Induction and Expansion of Human Pluripotent Stem Cell-Derived Retinal Pigment Epithelium},
  author={Grace Ellie Lidgerwood and Shiang Yong Lim and Duncan Edward Crombie and Ray Ali and Katherine P. Gill and Dami{\'a}n Rojo Hern{\'a}ndez and Josh Kie and A Limited Conquest and Hayley Shian Waugh and Raymond C. B. Wong and Helena Hai Liang and Alex W. Hewitt and Kathryn C. Davidson and Alice P{\'e}bay},
  journal={Stem Cell Reviews and Reports},
We demonstrate that a combination of Noggin, Dickkopf-1, Insulin Growth Factor 1 and basic Fibroblast Growth Factor, promotes the differentiation of human pluripotent stem cells into retinal pigment epithelium (RPE) cells. We describe an efficient one-step approach that allows the generation of RPE cells from both human embryonic stem cells and human induced pluripotent stem cells within 40–60 days without the need for manual excision, floating aggregates or imbedded cysts. Compared to methods… CONTINUE READING
This paper has been referenced on Twitter 1 time. VIEW TWEETS

Figures, Tables, and Topics from this paper.


Publications referenced by this paper.

Embryonic stem cell trials for macular degeneration: a preliminary report

  • Schwartz, D S.
  • Lancet
  • 2012
Highly Influential
3 Excerpts

Canonical/beta-catenin Wnt pathway activation improves retinal pigmented epithelium derivation from human embryonic stem cells

  • Leach, L L.
  • Investigative Ophthalmology & Visual Science,
  • 2015
1 Excerpt

Characterization of the retinal pigment epithelium in friedreich ataxia

  • Crombie, E D.
  • Biochemistry and Biophysics Reports,
  • 2015
1 Excerpt

Generation of a ciliary margin-like stem cell niche from self-organizing human retinal tissue

  • A Kuwahara
  • Nature Communications,
  • 2015

Characterization of human induced pluripotent stem cell-derived retinal pigment epithelium cell sheets aiming for clinical application

  • H Kamao
  • Stem Cell Reports,
  • 2014
1 Excerpt

Direct conversion of human fibroblasts into retinal pigment epithelium-like cells by defined factors

  • K Zhang
  • Protein & Cell,
  • 2014
1 Excerpt

Similar Papers

Loading similar papers…