Defined Medium Conditions for the Induction and Expansion of Human Pluripotent Stem Cell-Derived Retinal Pigment Epithelium

@article{Lidgerwood2015DefinedMC,
  title={Defined Medium Conditions for the Induction and Expansion of Human Pluripotent Stem Cell-Derived Retinal Pigment Epithelium},
  author={Grace Ellie Lidgerwood and Shiang Yong Lim and Duncan Edward Crombie and Ray Ali and Katherine P. Gill and Dami{\'a}n Rojo Hern{\'a}ndez and Josh Kie and A Limited Conquest and Hayley Shian Waugh and Raymond C. B. Wong and Helena Hai Liang and Alex W. Hewitt and Kathryn C. Davidson and Alice P{\'e}bay},
  journal={Stem Cell Reviews and Reports},
  year={2015},
  volume={12},
  pages={179-188}
}
We demonstrate that a combination of Noggin, Dickkopf-1, Insulin Growth Factor 1 and basic Fibroblast Growth Factor, promotes the differentiation of human pluripotent stem cells into retinal pigment epithelium (RPE) cells. We describe an efficient one-step approach that allows the generation of RPE cells from both human embryonic stem cells and human induced pluripotent stem cells within 40–60 days without the need for manual excision, floating aggregates or imbedded cysts. Compared to methods… CONTINUE READING
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