Defective retroviral endogenous RNA is efficiently transmitted by infectious particles produced on an avian retroviral vector packaging cell line.

@article{Ronfort1995DefectiveRE,
  title={Defective retroviral endogenous RNA is efficiently transmitted by infectious particles produced on an avian retroviral vector packaging cell line.},
  author={Corinne Ronfort and Anne Girod and Francois Loic Cosset and Catherine Legras and V. M. Nigon and Yahia Chebloune and G{\'e}rard Verdier},
  journal={Virology},
  year={1995},
  volume={207 1},
  pages={271-5}
}
This report describes the contamination of "helper-free" stocks of defective retroviral vector with particles bearing retroviral endogenous RNA. An avian leukosis virus-based packaging cell line was developed from LMH cells that bear the ev1, ev3, and ev6 retroviral endogenous loci. The results show that an endogenous retroviral transcript (ev3) was packaged into virions produced by this packaging cell line and was efficiently transferred along with the vector to target cells. The titer of the… CONTINUE READING