Defective mannosylation of glycosylphosphatidylinositol in Lec35 Chinese hamster ovary cells.

@article{Camp1993DefectiveMO,
  title={Defective mannosylation of glycosylphosphatidylinositol in Lec35 Chinese hamster ovary cells.},
  author={Laura A. Camp and Pinki Chauhan and Joshua D Farrar and Mark A. Lehrman},
  journal={The Journal of biological chemistry},
  year={1993},
  volume={268 9},
  pages={6721-8}
}
The Lec35 mutation (previously designated PIR) of Chinese hamster ovary cells is a recessive mutation that affects the participation of mannose-P-dolichol (MPD) in dolichol-P-P-oligosaccharide biosynthesis in vivo, even though MPD and the respective MPD-dependent mannosyltransferases are present. The Lec35 phenotype can be partially corrected by disrupting Lec35 cells and performing the transferase reactions in vitro, suggesting that the defect may be related to mislocalization of MPD. In this… CONTINUE READING