Defective Interfering DNAs of Plant Viruses

  title={Defective Interfering DNAs of Plant Viruses},
  author={Basavaprabhu L. Patil and Indranil Dasgupta},
  journal={Critical Reviews in Plant Sciences},
  pages={47 - 64}
Several plant DNA viruses produce significant quantities of deleted versions of their DNA in the infected plants, which is generally correlated with a slowing down of replication process of the viral DNA. These deleted versions of the viral DNA are called Defective-Interfering (DI) DNA because of their inhibitory effect on the helper virus. The sizes of the DI-DNAs for different plant viruses can vary from one tenth of the size of the viral genome to one half and are mostly encapsidated… 
Virus-mediated export of chromosomal DNA in plants
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Characterization and transient replication of tomato leaf curl virus defective DNAs
Mutational analysis showed the minimum sequence requirements for df DNA replication by TLCV to be the intergenic region containing the Rep-binding domains, which may indicate encapsidation.
Distinct evolutionary histories of the DNA-A and DNA-B components of bipartite begomoviruses
The analysis highlights that component exchange has played a far greater role in diversification of begomoviruses than previously suspected, although there are distinct differences in the apparent ability of different groups of viruses to utilize this "sexual" mechanism of genetic exchange.
Rad54 is not essential for any geminiviral replication mode in planta
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Diversity of Dicotyledenous-Infecting Geminiviruses and Their Associated DNA Molecules in Southern Africa, Including the South-West Indian Ocean Islands
This review aims to describe the emergence and diversification of various new begomoviral species and associated DI DNAs, in southern Africa, East Africa, and proximal Indian Ocean islands, which today threaten important vegetable and commercial crops.


Effects of Defective Interfering Viruses on Virus Replication and Pathogenesis In Vitro and In Vivo
Characterisation of DNA forms associated with cassava latent virus infection.
In addition to the major encapsidated DNA species found in preparations of cassava latent virus (genomic DNAs 1 and 2), there are minor DNA populations of twice (dimeric) and approximately half genome length that have an inhibitory effect on virus multiplication.
Defective and defective interfering RNAs of monopartite plus-strand RNA plant viruses.
The defective interfering (DI) RNAs represent one of several classes of symptom-modulating RNAs identified in association with RNA plant virus infections and may contain distinct types of modifications; however, the most prevalent is the deletion of one or more large segments of sequence.
Defective interfering RNAs and defective viruses associated with multipartite RNA viruses of plants
When selective conditions have been altered, multipartite RNA viruses can form defective viruses quite rapidly by deleting substantial portions of the viral genome, indicating that the entire viral genome is subject to continual selection pressure.
Geminiviruses: models for plant DNA replication, transcription, and cell cycle regulation.
Characterization of the Molecular Mechanism of Defective Interfering RNA-Mediated Symptom Attenuation in Tombusvirus-Infected Plants
The results presented suggest that DI RNA-mediated protection did not operate via the specific inhibition of 19-kDa protein expression but, more likely, DI RNAs in protective DI-helper virus combinations specifically interacted with viral products, preventing the induction of necrotic symptoms.
Defective viral DNA ameliorates symptoms of geminivirus infection in transgenic plants.
Transforming Nicotiana benthamiana with a single copy of a tandem repeat of subgenomic DNA B isolated from plants infected with a Kenyan isolate of the bipartite geminivirus African cassava mosaic virus might contribute to the production of plants showing increased tolerance to a number of economically important viral diseases.
Characterization of virus-specific DNA forms from tomato tissues infected by tobacco leaf curl virus : evidence for a single genomic component producing defective DNA molecules
A novel finding characterizing TLCV at the molecular level, from which it can be predicted as a distinct geminivirus possessing a single genomic component is found, in infected tomatoes, large amounts of a defective DNA accumulate that have retained cis-elements essential for replication.
Turnip crinkle virus defective interfering RNAs intensify viral symptoms and are generated de novo.
Defective interfering RNAs have now been found in association with a natural isolate of turnip crinkle virus (TCV-B) and are generated de novo after inoculation ofTurnip with virus derived from cloned transcripts.