Deep transcriptome sequencing reveals differences in global gene expression between normal and pale, soft, and exudative turkey meat.

@article{Malila2014DeepTS,
  title={Deep transcriptome sequencing reveals differences in global gene expression between normal and pale, soft, and exudative turkey meat.},
  author={Yuwares Malila and Kevin M. Carr and Catherine W. Ernst and Sandra G. Velleman and Kent M Reed and Gale M Strasburg},
  journal={Journal of animal science},
  year={2014},
  volume={92 3},
  pages={
          1250-60
        }
}
Previous studies from our laboratory suggested that differential expression of genes between normal and pale, soft, and exudative (PSE) turkey is associated with development of the PSE syndrome. However, a detailed understanding of molecular mechanisms responsible for the development of this meat defect remains unclear. The objective of this study was to extend and complement our previous work by using deep transcriptome RNA sequence analysis to compare the respective transcriptome profiles and… 
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References

SHOWING 1-10 OF 29 REFERENCES
Differential gene expression between normal and pale, soft, and exudative turkey meat.
TLDR
Substantial downregulation of pyruvate dehydrogenase kinase, isozyme 4 was observed in PSE samples, suggesting altered regulation of the aerobic metabolic pathway in the birds that developed PSE meat defect.
Identification of differentially expressed genes in chickens differing in muscle glycogen content and meat quality
TLDR
This study confirmed that, as in mammals, the down-regulation of CEBPB and RGS2 coincides with a decrease in peripheral adiposity in the chicken, but these genes are also suggested to affect muscle glycogen turnover through their role in the cAMP-dependent signalling pathway.
Progress in reducing the pale, soft and exudative (PSE) problem in pork and poultry meat.
Characterization of expressed sequence tags from turkey skeletal muscle.
TLDR
Sequences obtained in this study provide the basis for constructing a skeletal muscle-focused microarray, a tool that will facilitate the analysis of genes expressed during turkey muscle development, as well as the expression of genes underlying the genetic basis of muscle characteristics associated with meat quality.
Differential expression of calcium-regulating genes in heat-stressed turkey breast muscle is associated with meat quality.
TLDR
The data suggest that birds whose meat leads to PSE may fail to respond to heat stress appropriately due to a delay in the upregulation of the important calcium-regulating genes: αRYR, βR, and CASQ1.
Identification of two αRYR alleles and characterization of αRYR transcript variants in turkey skeletal muscle
Divergent mechanisms in generating molecular variations of αRYR and βRYR in turkey skeletal muscle
TLDR
The intriguing finding of this study is that αRYR and βRYR use completely divergent mechanisms to generate molecular variations that could affect calcium channel activity of either or both RYR isoforms.
Effect of rapid rigor mortis processes on protein functionality in pectoralis major muscle of domestic turkeys.
TLDR
The results indicate that irreversible myosin insolubility due to low pH and high-temperature conditions is decisive in the development of PSE turkey breast muscle.
Quantitative differential expression of alpha and beta ryanodine receptor genes in PSE (Pale, Soft, Exudative) meat from two chicken lines: broiler and layer
TLDR
These results provided the first evidence of PSE meat occurrence as a result of the differential expression of ryanodine receptor genes which might lead to an increased in Ca 2+ availability at the cell milieu.
The effect of heat stress on thyroid hormone response and meat quality in turkeys of two genetic lines.
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