Deep tissue two-photon microscopy

@article{Helmchen2005DeepTT,
  title={Deep tissue two-photon microscopy},
  author={Fritjof Helmchen and Winfried Denk},
  journal={Nature Methods},
  year={2005},
  volume={2},
  pages={932-940}
}
With few exceptions biological tissues strongly scatter light, making high-resolution deep imaging impossible for traditional—including confocal—fluorescence microscopy. Nonlinear optical microscopy, in particular two photon–excited fluorescence microscopy, has overcome this limitation, providing large depth penetration mainly because even multiply scattered signal photons can be assigned to their origin as the result of localized nonlinear signal generation. Two-photon microscopy thus allows… Expand
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