Deep tissue multiphoton microscopy using longer wavelength excitation.

  title={Deep tissue multiphoton microscopy using longer wavelength excitation.},
  author={Demirhan Kobat and Michael Durst and Nozomi Nishimura and Angela Wong and Chris Schaffer and Chris Xu},
  journal={Optics express},
  volume={17 16},
We compare the maximal two-photon fluorescence microscopy (TPM) imaging depth achieved with 775-nm excitation to that achieved with 1280-nm excitation through in vivo and ex vivo TPM of fluorescently-labeled blood vessels in mouse brain. We achieved high contrast imaging of blood vessels at approximately twice the depth with 1280-nm excitation as with 775-nm excitation. An imaging depth of 1 mm can be achieved in in vivo imaging of adult mouse brains at 1280 nm with approximately 1-nJ pulse… CONTINUE READING


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