Deep sequencing of a dimethylsulfoniopropionate-degrading gene (dmdA) by using PCR primer pairs designed on the basis of marine metagenomic data.

@article{Varaljay2010DeepSO,
  title={Deep sequencing of a dimethylsulfoniopropionate-degrading gene (dmdA) by using PCR primer pairs designed on the basis of marine metagenomic data.},
  author={Vanessa A. Varaljay and Erinn C Howard and Shulei Sun and Mary Ann Moran},
  journal={Applied and environmental microbiology},
  year={2010},
  volume={76 2},
  pages={609-17}
}
In silico design and testing of environmental primer pairs with metagenomic data are beneficial for capturing a greater proportion of the natural sequence heterogeneity in microbial functional genes, as well as for understanding limitations of existing primer sets that were designed from more restricted sequence data. PCR primer pairs targeting 10 environmental clades and subclades of the dimethylsulfoniopropionate (DMSP) demethylase protein, DmdA, were designed using an iterative bioinformatic… CONTINUE READING