Deep sequencing and variant analysis of an Italian pathogenic field strain of equine infectious anaemia virus.

@article{Cappelli2017DeepSA,
  title={Deep sequencing and variant analysis of an Italian pathogenic field strain of equine infectious anaemia virus.},
  author={Katia Cappelli and Royer F Cook and Valentina Stefanetti and Fabrizio Passamonti and Gian Luca Autorino and Maria Teresa Scicluna and Mauro Coletti and Andrea Verini Supplizi and Stefano Capomaccio},
  journal={Transboundary and emerging diseases},
  year={2017},
  volume={64 6},
  pages={
          2104-2112
        }
}
Equine infectious anaemia virus (EIAV) is a lentivirus with an almost worldwide distribution that causes persistent infections in equids. Technical limitations have restricted genetic analysis of EIAV field isolates predominantly to gag sequences resulting in very little published information concerning the extent of inter-strain variation in pol, env and the three ancillary open reading frames (ORFs). Here, we describe the use of long-range PCR in conjunction with next-generation sequencing… CONTINUE READING
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