Deep manifold learning reveals hidden dynamics of proteasome autoregulation

  title={Deep manifold learning reveals hidden dynamics of proteasome autoregulation},
  author={Zhaolong Wu and Shuwen Zhang and W. Wang and Yin-ping Ma and Yuanchen Dong and Youdong Mao},
The 2.5-MDa 26S proteasome maintains proteostasis and regulates myriad cellular processes. How polyubiquitylated substrate interactions regulate proteasome activity is not understood. Here we introduce a deep manifold learning framework, named AlphaCryo4D, which enables atomic-level cryogenic electron microscopy (cryo-EM) reconstructions of nonequilibrium conformational continuum and reconstitutes hidden dynamics of proteasome autoregulation in the act of substrate degradation. AlphaCryo4D… Expand

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