Deep knot structure for construction of active site and cofactor binding site of tRNA modification enzyme.

@article{Nureki2004DeepKS,
  title={Deep knot structure for construction of active site and cofactor binding site of tRNA modification enzyme.},
  author={Osamu Nureki and Kazunori Watanabe and Shuya Fukai and Ryohei Ishii and Yaeta Endo and Hiroyuki Hori and Shigeyuki Yokoyama},
  journal={Structure},
  year={2004},
  volume={12 4},
  pages={593-602}
}
The tRNA(Gm18) methyltransferase (TrmH) catalyzes the 2'-O methylation of guanosine 18 (Gua18) of tRNA. We solved the crystal structure of Thermus thermophilus TrmH complexed with S-adenosyl-L-methionine at 1.85 A resolution. The catalytic domain contains a deep trefoil knot, which mutational analyses revealed to be crucial for the formation of the… CONTINUE READING