Deep freezing of horse embryos.

  title={Deep freezing of horse embryos.},
  author={M Czlonkowska and M S Boyle and William R. Allen},
  journal={Journal of reproduction and fertility},
  volume={75 2},
Fourteen horse embryos recovered non-surgically on Days 6-8 after ovulation (Day 0) were cooled slowly to - 35 degrees C (7 embryos) or - 40 degrees C (7 embryos) and stored in liquid nitrogen (- 196 degrees C) for 4-98 days. Surgical transfer of the thawed embryos to unmated recipient mares that had ovulated - 2 to + 1 days with respect to the embryo donors resulted initially in the establishment of 4 conceptuses. However, only one mare maintained her pregnancy to term. 


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