Deep UV plasmonic enhancement of single protein autofluorescence in zero-mode waveguides.

@article{Barulin2019DeepUP,
  title={Deep UV plasmonic enhancement of single protein autofluorescence in zero-mode waveguides.},
  author={Aleksandr Barulin and Jean-Beno{\^i}t Claude and Satyajit Patra and Nicolas Bonod and J{\'e}r{\^o}me Wenger},
  journal={Nano letters},
  year={2019}
}
Single molecule detection provides detailed information about molecular structures and functions, but it generally requires the presence of a fluorescent marker which can interfere with the activity of the target molecule or complicate the sample production. Detecting a single protein with its natural UV autofluorescence is an attractive approach to avoid all the issues related to fluorescence labelling. However, the UV autofluorescence signal from a single protein is generally extremely weak… Expand
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References

SHOWING 1-10 OF 85 REFERENCES
Bright unidirectional fluorescence emission of molecules in a nanoaperture with plasmonic corrugations.
TLDR
It is reported that gold nanoapertures surrounded by periodic corrugations transform standard fluorescent molecules into bright unidirectional sources and the increased light-matter coupling as well as the radiation pattern intensity is quantified. Expand
Plasmonic antennas and zero-mode waveguides to enhance single molecule fluorescence detection and fluorescence correlation spectroscopy toward physiological concentrations.
TLDR
Single molecule spectroscopy techniques greatly benefit from ZMW and plasmonic antennas to enter a new dimension of molecular concentration reaching physiological conditions, and is promising to reveal new insights on biological functions and dynamics. Expand
Resonant plasmonic enhancement of single-molecule fluorescence by individual gold nanorods.
TLDR
Study of fluorescence enhancement by isolated gold nanorods and the role of the surface plasmon resonance (SPR) on the observed enhancements, which are consistent with numerical simulations. Expand
Dark-Field Illumination on Zero-Mode Waveguide/Microfluidic Hybrid Chip Reveals T4 Replisomal Protein Interactions
TLDR
Conical lens-based dark-field fluorescence microscopy in combination with a ZMW/microfluidic chip for single-molecule fluorescence imaging is presented and it is demonstrated that compared to epi-illumination, the dark- field configuration displayed diminished background and noise and enhanced signal-to-noise ratios. Expand
Distance-Dependent Metal-Enhanced Intrinsic Fluorescence of Proteins Using Polyelectrolyte Layer-by-Layer Assembly and Aluminum Nanoparticles.
TLDR
The present study indicates the distance dependence nature of metal-enhanced intrinsic fluorescence of proteins and potential of LbL assembly to control the metal-to-fluorophore distance in the UV wavelength region. Expand
Nanoaperture-enhanced fluorescence : Towards higher detection rates with plasmonic metals
A bare nanometric aperture milled in a metallic film forms a simple nanophotonic device that can strongly enhance the optical properties of nearby emitters such as fluorescent molecules. In thisExpand
Label-Free Optical Detection of DNA Translocations through Plasmonic Nanopores
TLDR
It is demonstrated that translocations of single DNA molecules can be optically detected in the transmitted light intensity through an inverted-bowtie plasmonic nanopore, and this label-free optical detection scheme offers opportunities to probe native DNA–protein interactions at physiological conditions. Expand
Plasmonic zero mode waveguide for highly confined and enhanced fluorescence emission.
TLDR
A plasmonic nanoslot that is capable of performing enhanced single molecule detection at 10 μM concentrations is fabricated, which yields a 25-fold fluorescence enhancement and is compared with a standard aluminum zero-mode waveguide. Expand
Two-photon excitation microscopy of tryptophan-containing proteins
TLDR
It was discovered that the population of the oxygenated state of hemocyanin can be changed by means of a one- photon process with the same laser source that monitors this population in a two-photon process. Expand
In-Plane Plasmonic Antenna Arrays with Surface Nanogaps for Giant Fluorescence Enhancement.
TLDR
A new nanofabrication technique is described that applies planarization, etch back, and template stripping to expose the excitation hotspot at the surface, providing a major improvement over conventional electron beam lithography methods. Expand
...
1
2
3
4
5
...