Deep UV plasmonic enhancement of single protein autofluorescence in zero-mode waveguides.

  title={Deep UV plasmonic enhancement of single protein autofluorescence in zero-mode waveguides.},
  author={Aleksandr Barulin and Jean-Beno{\^i}t Claude and Satyajit Patra and Nicolas Bonod and J{\'e}r{\^o}me Wenger},
  journal={Nano letters},
Single molecule detection provides detailed information about molecular structures and functions, but it generally requires the presence of a fluorescent marker which can interfere with the activity of the target molecule or complicate the sample production. Detecting a single protein with its natural UV autofluorescence is an attractive approach to avoid all the issues related to fluorescence labelling. However, the UV autofluorescence signal from a single protein is generally extremely weak… Expand
11 Citations
Purcell radiative rate enhancement of label-free proteins with ultraviolet aluminum plasmonics
The vast majority of proteins are intrinsically fluorescent in the ultraviolet, thanks to the emission from their tryptophan and tyrosine amino-acid constituents. However, the proteinExpand
Ultraviolet Photostability Improvement for Autofluorescence Correlation Spectroscopy on Label-free Proteins.
Combining enzymatic oxygen scavengers with antioxidants and triplet state quenchers greatly promotes the protein photostability, reduces the photobleaching probability and improves the net autofluorescence detection rate, and opens new perspectives to improve the UV durability of organic devices. Expand
UV fluorescence enhancement by aluminum and magnesium equilateral bowtie nanoantennas
The intrinsic fluorescence of biomolecules such as proteins and nucleic acids lies in the ultraviolet (UV) range of the spectrum. UV plasmonic nano-structures have been shown to enhance theExpand
Zero-mode waveguides can be made better: fluorescence enhancement with rectangular aluminum nanoapertures from the visible to the deep ultraviolet
Nanoapertures milled in metallic films called zero-mode waveguides (ZMWs) overcome the limitations of classical confocal microscopes by enabling single molecule analysis at micromolar concentrationsExpand
Surface passivation of zero-mode waveguide nanostructures: benchmarking protocols and fluorescent labels
The nonspecific adhesion of DNA double strands onto the ZMW surface is directly mediated by the organic fluorescent dye being used, as Atto 550 and Atto 647N show a pronounced tendency to adhere to the Z MW while the Alexa Fluor 546 and 647 are remarkably free of this effect. Expand
Near-UV luminescence tomography with an aperture-free meta super oscillatory lens for single molecule detection.
  • Hassan Ali, Haibin Ni, X. Xu
  • Medicine, Physics
  • Journal of the Optical Society of America. A, Optics, image science, and vision
  • 2020
A negative-indexed permeability-controlled meta SOL is proposed, which restores the light concentration via exciting surface plasmon resonance and enhances luminescence at the region of interest, i.e., fluid sample, and will offer a new and easy approach to uplift the efficiency of super oscillatory lenses in the near-UV regime to benefit single molecule detection techniques and thus the novelty. Expand
Quantum Yield Limits for the Detection of Single-Molecule Fluorescence Enhancement by a Gold Nanorod
Fluorescence-based single-molecule optical detection techniques are widely chosen over other methods, owing to the ease of background screening and better signal-to-noise throughput. Nonetheless, t...
FRET-based dynamic structural biology: Challenges, perspectives and an appeal for open-science practices
The current ‘state of the art’ of smFRET from different perspectives is described, points to unresolved methodological issues for quantitative structural studies, provides a set of ‘soft recommendations’ about which an emerging consensus exists, and lists openly available resources for newcomers and seasoned practitioners. Expand
Recent advances in plasmonic nanocavities for single-molecule spectroscopy
This mini-review reports the most recent results on plasmonic nanocavities applied to enhanced single-molecule detection and spectroscopy.


Bright unidirectional fluorescence emission of molecules in a nanoaperture with plasmonic corrugations.
It is reported that gold nanoapertures surrounded by periodic corrugations transform standard fluorescent molecules into bright unidirectional sources and the increased light-matter coupling as well as the radiation pattern intensity is quantified. Expand
Plasmonic antennas and zero-mode waveguides to enhance single molecule fluorescence detection and fluorescence correlation spectroscopy toward physiological concentrations.
Single molecule spectroscopy techniques greatly benefit from ZMW and plasmonic antennas to enter a new dimension of molecular concentration reaching physiological conditions, and is promising to reveal new insights on biological functions and dynamics. Expand
Resonant plasmonic enhancement of single-molecule fluorescence by individual gold nanorods.
Study of fluorescence enhancement by isolated gold nanorods and the role of the surface plasmon resonance (SPR) on the observed enhancements, which are consistent with numerical simulations. Expand
Dark-Field Illumination on Zero-Mode Waveguide/Microfluidic Hybrid Chip Reveals T4 Replisomal Protein Interactions
Conical lens-based dark-field fluorescence microscopy in combination with a ZMW/microfluidic chip for single-molecule fluorescence imaging is presented and it is demonstrated that compared to epi-illumination, the dark- field configuration displayed diminished background and noise and enhanced signal-to-noise ratios. Expand
Distance-Dependent Metal-Enhanced Intrinsic Fluorescence of Proteins Using Polyelectrolyte Layer-by-Layer Assembly and Aluminum Nanoparticles.
The present study indicates the distance dependence nature of metal-enhanced intrinsic fluorescence of proteins and potential of LbL assembly to control the metal-to-fluorophore distance in the UV wavelength region. Expand
Nanoaperture-enhanced fluorescence : Towards higher detection rates with plasmonic metals
A bare nanometric aperture milled in a metallic film forms a simple nanophotonic device that can strongly enhance the optical properties of nearby emitters such as fluorescent molecules. In thisExpand
Label-Free Optical Detection of DNA Translocations through Plasmonic Nanopores
It is demonstrated that translocations of single DNA molecules can be optically detected in the transmitted light intensity through an inverted-bowtie plasmonic nanopore, and this label-free optical detection scheme offers opportunities to probe native DNA–protein interactions at physiological conditions. Expand
Plasmonic zero mode waveguide for highly confined and enhanced fluorescence emission.
A plasmonic nanoslot that is capable of performing enhanced single molecule detection at 10 μM concentrations is fabricated, which yields a 25-fold fluorescence enhancement and is compared with a standard aluminum zero-mode waveguide. Expand
Two-photon excitation microscopy of tryptophan-containing proteins
It was discovered that the population of the oxygenated state of hemocyanin can be changed by means of a one- photon process with the same laser source that monitors this population in a two-photon process. Expand
In-Plane Plasmonic Antenna Arrays with Surface Nanogaps for Giant Fluorescence Enhancement.
A new nanofabrication technique is described that applies planarization, etch back, and template stripping to expose the excitation hotspot at the surface, providing a major improvement over conventional electron beam lithography methods. Expand