Deep Sequencing of Mixed Total DNA without Barcodes Allows Efficient Assembly of Highly Plastic Ascidian Mitochondrial Genomes

@inproceedings{Rubinstein2013DeepSO,
  title={Deep Sequencing of Mixed Total DNA without Barcodes Allows Efficient Assembly of Highly Plastic Ascidian Mitochondrial Genomes},
  author={Nimrod D. Rubinstein and Tamar Feldstein and Noa Shenkar and Fidel Botero-Castro and Francesca Griggio and Francesco Mastrototaro and Fr{\'e}d{\'e}ric Delsuc and Emmanuel J. P. Douzery and Carmela Gissi and Doroth{\'e}e Huchon},
  booktitle={Genome biology and evolution},
  year={2013}
}
Ascidians or sea squirts form a diverse group within chordates, which includes a few thousand members of marine sessile filter-feeding animals. Their mitochondrial genomes are characterized by particularly high evolutionary rates and rampant gene rearrangements. This extreme variability complicates standard polymerase chain reaction (PCR) based techniques for molecular characterization studies, and consequently only a few complete Ascidian mitochondrial genome sequences are available. Using the… CONTINUE READING

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