Deep Mutational Scanning: Calculating Enrichment Scores for Protein Variants from DNA Sequencing Output Files.

Abstract

During a deep mutational scanning experiment, a collection of variants of a given protein is subjected to high-throughput sequencing before and after selection. The variants that perform well during selection will increase in abundance, whereas those that perform poorly will decrease. Generating a sequence-function map of a protein from a deep mutational… (More)
DOI: 10.1101/pdb.prot085233

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Cite this paper

@article{Starita2015DeepMS, title={Deep Mutational Scanning: Calculating Enrichment Scores for Protein Variants from DNA Sequencing Output Files.}, author={Lea M. Starita and Stanley Fields}, journal={Cold Spring Harbor protocols}, year={2015}, volume={2015 8}, pages={781-3} }