Deep Amino Acid Sequencing of Native Brain GABAA Receptors Using High-Resolution Mass Spectrometry*

@inproceedings{Chen2012DeepAA,
  title={Deep Amino Acid Sequencing of Native Brain GABAA Receptors Using High-Resolution Mass Spectrometry*},
  author={Zi-Wei Chen and Karoline Fuchs and Werner Sieghart and Raymond R. Townsend and Alex S Evers},
  booktitle={Molecular & cellular proteomics : MCP},
  year={2012}
}
Mass spectrometric sequencing of low abundance, integral membrane proteins, particularly the transmembrane domains, presents challenges that span the multiple phases of sample preparation including solubilization, purification, enzymatic digestion, peptide extraction, and chromatographic separation. We describe a method through which we have obtained high peptide coverage for 12 γ-aminobutyric acid type A receptor (GABAA receptor) subunits from 2 picomoles of affinity-purified GABAA receptors… CONTINUE READING

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