Decreased protamine-1 transcript levels in testes from infertile men.

@article{Steger2003DecreasedPT,
  title={Decreased protamine-1 transcript levels in testes from infertile men.},
  author={Klaus Steger and Ludger Fink and Klaus Failing and Rainer Bohle and Sabine Kliesch and Wolfgang Weidner and Martin W Bergmann},
  journal={Molecular human reproduction},
  year={2003},
  volume={9 6},
  pages={331-6}
}
Infertile men exhibit an aberrant protamine-1 (Prm1) to protamine-2 (Prm2) ratio at both the mRNA and protein level. We therefore investigated whether male infertility could be related to the amount of Prm1 and Prm2 mRNA by applying real time quantitative PCR following RNA extraction from routinely Bouin-fixed and paraffin-embedded testicular biopsies. Samples (n = 51) were normalized to the same amount and similar size of tissue sections. The threshold cycle (C(T)) representing a measure of… CONTINUE READING

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