Decreased NADH glutamate synthase activity in nodules and flowers of alfalfa (Medicago sativa L.) transformed with an antisense glutamate synthase transgene.

@article{Schoenbeck2000DecreasedNG,
  title={Decreased NADH glutamate synthase activity in nodules and flowers of alfalfa (Medicago sativa L.) transformed with an antisense glutamate synthase transgene.},
  author={Mark A. Schoenbeck and Stephen J. Temple and Gian Baptista Trepp and J. M. Blumenthal and Deborah A Samac and J. Stephen Gantt and Germ{\'a}n Hern{\'a}ndez and Carroll P. Vance},
  journal={Journal of experimental botany},
  year={2000},
  volume={51 342},
  pages={
          29-39
        }
}
Legumes obtain a substantial portion of their nitrogen (N) from symbiotic N2 fixation in root nodules. The glutamine synthetase (GS, EC 6.3.1.2)/glutamate synthase (GOGAT) cycle is responsible for the initial N assimilation. This report describes the analysis of a transgenic alfalfa (Medicago sativa L.) line containing an antisense NADH-GOGAT (EC 1.4.1.14) under the control of the nodule-enhanced aspartate amino-transferase (AAT-2) promoter. In one transgenic line, NADH-GOGAT enzyme activity… CONTINUE READING

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