Deconvolution of fluorescence lifetime imaging microscopy (FLIM)

  title={Deconvolution of fluorescence lifetime imaging microscopy (FLIM)},
  author={Varun Mannam and Xiaotong Yuan and Scott Howard},
Fluorescence lifetime imaging microscopy (FLIM) is an important technique to understand the chemical microenvironment in cells and tissues since it provides additional contrast compared to conventional fluorescence imaging. When two fluorophores within a diffraction limit are excited, the resulting emission leads to nonlinear spatial distortion and localization effects in intensity (magnitude) and lifetime (phase) components. To address this issue, in this work, we provide a theoretical model… 


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[Fluorescence microscopy].
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