Deconvolution and chromatic aberration corrections in quantifying colocalization of a transcription factor in three-dimensional cellular space.

Abstract

In the realm of multi-dimensional confocal microscopy, colocalization analysis of fluorescent emission signals has proven to be an invaluable tool for detecting molecular interactions between biological macromolecules at the subcellular level. We show here that image processing operations such as the deconvolution and chromatic corrections play a crucial… (More)
DOI: 10.1016/j.micron.2010.03.005

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@article{Abraham2010DeconvolutionAC, title={Deconvolution and chromatic aberration corrections in quantifying colocalization of a transcription factor in three-dimensional cellular space.}, author={Thomas Abraham and Sarah E. Allan and Megan K. Levings}, journal={Micron}, year={2010}, volume={41 6}, pages={633-40} }