Decontamination methods for samples preserved in cetylpyridinium chloride and cultured on thin-layer agar.

@article{Ardizzoni2014DecontaminationMF,
  title={Decontamination methods for samples preserved in cetylpyridinium chloride and cultured on thin-layer agar.},
  author={Elisa Ardizzoni and Wim Mulders and Elisabeth S{\'a}nchez-Padilla and Francis F V Varaine and B. C. de Jong and Leen Rigouts},
  journal={The international journal of tuberculosis and lung disease : the official journal of the International Union against Tuberculosis and Lung Disease},
  year={2014},
  volume={18 8},
  pages={972-7}
}
SETTING Long transportation times of samples to culture laboratories can lead to higher contamination rates and significant loss of viability, resulting in lower culture positivity rates. Thin-layer agar (TLA) is a sensitive culture method for the isolation of Mycobacterium tuberculosis that has been optimised with N-acetyl-L-cysteine-sodium hydroxide (NALC-NaOH) decontaminated samples. The combination of the TLA culture method and other decontamination procedures has not been extensively… CONTINUE READING
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