Deconstructing Retinal Organoids: Single Cell RNA-Seq Reveals the Cellular Components of Human Pluripotent Stem Cell-Derived Retina
@inproceedings{oseph2019DeconstructingRO, title={Deconstructing Retinal Organoids: Single Cell RNA-Seq Reveals the Cellular Components of Human Pluripotent Stem Cell-Derived Retina}, author={oseph and ollin and Achel and UEEN and IRTHE and ORGAU and Onathan and OXHEAD and Imon and OCKELL}, year={2019} }
The rapid improvements in single cell sequencing technologies and analyses afford greater scope for dissecting organoid cultures composed of multiple cell types and create an opportunity to interrogate these models to understand tissue biology, cellular behavior and interactions. To this end, retinal organoids generated from human embryonic stem cells (hESCs) were analyzed by single cell RNA-sequencing (scRNA-Seq) at 3 time points of differentiation. Combinatorial data from all time points… CONTINUE READING
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Retinogenesis of the Human Fetal Retina: An Apical Polarity Perspective
- Biology, Medicine
- Genes
- 2019
- 6
- PDF
References
SHOWING 1-10 OF 36 REFERENCES
Isolation and Comparative Transcriptome Analysis of Human Fetal and iPSC-Derived Cone Photoreceptor Cells
- Biology, Medicine
- Stem cell reports
- 2017
- 49
- PDF
The transcriptome of retinal Müller glial cells
- Biology, Medicine
- The Journal of comparative neurology
- 2008
- 283
- PDF
Retinal pigment epithelium development, plasticity, and tissue homeostasis.
- Biology, Medicine
- Experimental eye research
- 2014
- 131
Development and programed cell death in the mammalian eye.
- Biology, Medicine
- The International journal of developmental biology
- 2015
- 16
- PDF
Genetic analysis of the homeodomain transcription factor Chx10 in the retina using a novel multifunctional BAC transgenic mouse reporter.
- Biology, Medicine
- Developmental biology
- 2004
- 256
Timing and topography of cell genesis in the rat retina
- Biology, Medicine
- The Journal of comparative neurology
- 2004
- 320
- PDF
Self-formation of optic cups and storable stratified neural retina from human ESCs.
- Biology, Medicine
- Cell stem cell
- 2012
- 839
- PDF
Highly Parallel Genome-wide Expression Profiling of Individual Cells Using Nanoliter Droplets
- Chemistry, Biology
- Cell
- 2015
- 3,337
- PDF
Self-organizing optic-cup morphogenesis in three-dimensional culture
- Biology
- Neuroscience Research
- 2011
- 248
The dynamics and regulators of cell fate decisions are revealed by pseudotemporal ordering of single cells
- Biology, Medicine
- Nature Biotechnology
- 2014
- 2,046