Decoding the oak genome: public release of sequence data, assembly, annotation and publication strategies

@article{Plomion2016DecodingTO,
  title={Decoding the oak genome: public release of sequence data, assembly, annotation and publication strategies},
  author={C. Plomion and J. Aury and J. Amselem and Tina Alaeitabar and V. Barbe and Caroline Belser and H. Berg{\`e}s and C. Bod{\'e}n{\`e}s and N. Boudet and C. Boury and A. Canaguier and A. Couloux and Corinne Da Silva and S. Duplessis and F. Ehrenmann and Barbara Estrada-Mairey and St{\'e}phanie Fouteau and Nicolas Francillonne and C. Gaspin and C. Guichard and C. Klopp and K. Labadie and C. Lalanne and I. Le Clainche and J. Lepl{\'e} and G. Le Provost and Thibault Leroy and I. Lesur and F. Martin and Jonathan Mercier and C. Michotey and F. Murat and F. Salin and D. Steinbach and P. Faivre-Rampant and P. Wincker and J. Salse and H. Quesneville and A. Kremer},
  journal={Molecular Ecology Resources},
  year={2016},
  volume={16}
}
The 1.5 Gbp/2C genome of pedunculate oak (Quercus robur) has been sequenced. A strategy was established for dealing with the challenges imposed by the sequencing of such a large, complex and highly heterozygous genome by a whole‐genome shotgun (WGS) approach, without the use of costly and time‐consuming methods, such as fosmid or BAC clone‐based hierarchical sequencing methods. The sequencing strategy combined short and long reads. Over 49 million reads provided by Roche 454 GS‐FLX technology… Expand
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