Decoding in the absence of a codon by tmRNA and SmpB in the ribosome.

Abstract

In bacteria, ribosomes stalled at the end of truncated messages are rescued by transfer-messenger RNA (tmRNA), a bifunctional molecule that acts as both a transfer RNA (tRNA) and a messenger RNA (mRNA), and SmpB, a small protein that works in concert with tmRNA. Here, we present the crystal structure of a tmRNA fragment, SmpB and elongation factor Tu bound to the ribosome at 3.2 angstroms resolution. The structure shows how SmpB plays the role of both the anticodon loop of tRNA and portions of mRNA to facilitate decoding in the absence of an mRNA codon in the A site of the ribosome and explains why the tmRNA-SmpB system does not interfere with normal translation.

DOI: 10.1126/science.1217039

Extracted Key Phrases

3 Figures and Tables

02040201220132014201520162017
Citations per Year

126 Citations

Semantic Scholar estimates that this publication has 126 citations based on the available data.

See our FAQ for additional information.

Cite this paper

@article{Neubauer2012DecodingIT, title={Decoding in the absence of a codon by tmRNA and SmpB in the ribosome.}, author={Cajetan Neubauer and Reynald Gillet and Ann C. Kelley and V Ramakrishnan}, journal={Science}, year={2012}, volume={335 6074}, pages={1366-9} }