Deciphering the catalytic machinery in a universally conserved ribosome binding ATPase YchF.

@article{Tomar2011DecipheringTC,
  title={Deciphering the catalytic machinery in a universally conserved ribosome binding ATPase YchF.},
  author={Sushil Kumar Tomar and P. Saidesh Kumar and B S Jai Prakash},
  journal={Biochemical and biophysical research communications},
  year={2011},
  volume={408 3},
  pages={459-64}
}
YchF, a universally conserved protein, hitherto thought to be a GTPase, was shown to be an ATPase based on structural and biochemical studies on hOLA1, a human ortholog of YchF. However, the cellular role of YchF is unclear. Based on the presence of a RNA binding domain in this protein and significant homology to ribosome binding Obg family GTPases, we examined its ability to associate with the ribosome. Here, we show that Escherichia coli YchF binds the 50S and 70S ribosomal particles in a… CONTINUE READING