Deamination of AMP and adenosine and dephosphorylation of AMP in subcellular structures of the myocardium in experimental aortic stenosis

Abstract

The left ventr icle was cut up finely with s c i s so r s and then homogenized for 1-2 min with 4.5 volumes of 0.25 M sucrose solution. Cell f ract ions were isolated f rom the homogenate by differential centrifugation: the myof ib r i l l a ry -nuc lea r f ract ion at 600 g (the upper layer of the residue was removed), mitochondria at 3500 g, and mic rosomes at 75,000 g. The f i r s t two fract ions were washed twice with 0.25 M sucrose solution.

DOI: 10.1007/BF00810796

Cite this paper

@article{Nechiporenko2004DeaminationOA, title={Deamination of AMP and adenosine and dephosphorylation of AMP in subcellular structures of the myocardium in experimental aortic stenosis}, author={Z. Yu. Nechiporenko and E. G. Goncharenko}, journal={Bulletin of Experimental Biology and Medicine}, year={2004}, volume={67}, pages={146-148} }