Deactivation of formate dehydrogenase (FDH) in solution and at gas-liquid interfaces.

@article{Bommarius2005DeactivationOF,
  title={Deactivation of formate dehydrogenase (FDH) in solution and at gas-liquid interfaces.},
  author={Andreas S. Bommarius and Andreas Karau},
  journal={Biotechnology progress},
  year={2005},
  volume={21 6},
  pages={1663-72}
}
Enzymes, increasingly important in the synthesis of fine chemicals and pharmaceutical intermediates, are often insufficiently stable under reacting conditions. We have investigated the stability, in homogeneous aqueous solution and at gas-liquid interfaces, of formate dehydrogenase (FDH), important for cofactor regeneration, from Candida boidinii and overexpressed in E. coli. When exposed to mechanical stress, residual activity, [E](t)/[E](0), and residual protein were found to scale… CONTINUE READING