Deactivation mechanisms of chloroperoxidase during biotransformations.

  title={Deactivation mechanisms of chloroperoxidase during biotransformations.},
  author={Jin-Byung Park and Douglas S Clark},
  journal={Biotechnology and bioengineering},
  volume={93 6},
Inactivation mechanisms of chloroperoxidase (CPO) from Caldariomyces fumago have been investigated with the aim of improving the practical utility of CPO for hydrocarbon oxidation. Deactivation studies in the presence of oxidants (i.e., hydrogen peroxide and t-butyl hydroperoxide) indicated that CPO lost oxidation activity toward hydrocarbon substrates during dismutation of hydrogen peroxide. The loss of enzyme activity was accompanied by the apparent destruction of the heme rather than… CONTINUE READING