Deacetoxycephalosporin C synthetase and deacetoxycephalosporin C hydroxylase are two separate enzymes in Streptomyces clavuligerus.

@article{Jensen1985DeacetoxycephalosporinCS,
  title={Deacetoxycephalosporin C synthetase and deacetoxycephalosporin C hydroxylase are two separate enzymes in Streptomyces clavuligerus.},
  author={Susan E. Jensen and Donald W. S. Westlake and S. K. Wolfe},
  journal={The Journal of antibiotics},
  year={1985},
  volume={38 2},
  pages={263-5}
}
pounds is accomplished by means of a biosynthetic pathway which closely parallels the corresponding pathway to cephalosporin C in Cephalosporium acremonium. Two of the enzymes involved in this pathway, deacetoxycephalosporin C (DAOC) synthetase and DAOC hydroxylase, carry out sequential reactions in which penicillin N undergoes ring expansion to DAOC and then DAOC is hydroxylated at the C3 methyl group to give deacetylcephalosporin C (DAC). These two enzymes are intermolecular dioxygenases… CONTINUE READING

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References

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Showing 1-6 of 6 references

NUESCH: Partial purification and catalytic properties of a bifunctional enzyme in the biosynthetic pathway of,S-lactams in Cephalosporium acremonium

  • T KUENZIM.
  • SCHEIDEGGER,
  • 1984

DEMAIN: Partial purification and properties of the a-ketoglutarate-linked ring-expansion enzyme of 3-lactam biosynthesis of Cephalosporitan acremoniwn

  • J. KUPKA, S. WOLFE Y.-Q. SHEN, L A.
  • FEMS Microbiol. Lett
  • 1983

WOLFE: Analysis of penicillin N ring expansion activity from Streptomyces clavuligerus by ion-pair highpressure liquid chromatography

  • JENSEN, E S., D.W.S. WESTLAKE
  • Antimicrob. Agents Chemother
  • 1983

WOLFE : Cephalosporin formation by cellfree extracts from Streptomyces clavuligerus

  • S. E. JENSEN, R. J. BOWERS D.W.S. WESTLAKE
  • J. Antibiotics
  • 1982

WEHRLI: Investigation of the two final steps in the biosynthesis of cephalosporin C using permeabilized cells of Cephalosporium acremonium

  • FELIX, R H., J. NUESCH
  • FEMS M icrobiol. Lett
  • 1980

MORIN: Stimulation of the conversion of penicillin N to cephalosporin by ascorbic acid, (Y-ketoglutarate and ferrous ion in cell-free extracts of strains of Cephalosporium acremoniumn

  • D. J. HOOK, R. P. ELANDER L.T. CHANG, B R.
  • Biochem. Biophys. Res. Commun
  • 1979

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