De novo synthesis of adenine nucleotides in different skeletal muscle fiber types.

  title={De novo synthesis of adenine nucleotides in different skeletal muscle fiber types.},
  author={Peter C. Tullson and Henry B John-Alder and David A. Hood and R. L. Terjung},
  journal={The American journal of physiology},
  volume={255 3 Pt 1},
Management of adenine nucleotide catabolism differs among skeletal muscle fiber types. This study evaluated whether there are corresponding differences in the rates of de novo synthesis of adenine nucleotide among fiber type sections of skeletal muscle using an isolated perfused rat hindquarter preparation. Label incorporation into adenine nucleotides from the [1-14C]glycine precursor was determined and used to calculate synthesis rates based on the intracellular glycine specific radioactivity… 

Purine salvage to adenine nucleotides in different skeletal muscle fiber types.

In the absence of ribose supplementation, purine salvage rates are relatively low, especially compared with the AdN pool size in skeletal muscle, consistent with tissue 5-phosphoribosyl-1-pyrophosphate being rate limiting for Purine salvage.

Influence of ribose on adenine salvage after intense muscle contractions.

Data indicate that adenine salvage rates were essentially unaltered during recovery from intense contractions, and ribose supplementation did not affect subsequent muscle force production after 60 min of recovery.

Phosphate uptake and PiT-1 protein expression in rat skeletal muscle.

Skeletal muscle fiber types differ in their contents of total phosphate, which includes inorganic phosphate (P(i)) and high-energy organic pools of ATP and phosphocreatine (PCr) and fractional turnover of the cellular P(i) pool varies among fiber types, indicating differential management of intracellular P( i), likely due to differences in resistance to P(o) efflux from the fiber.

Phosphate uptake in rat skeletal muscle is reduced during isometric contractions.

The data imply that P( i) uptake in skeletal muscle is acutely modulated during contractions and that decreases in P(i) uptake rates, in combination with expected increases in P (i) efflux, exacerbate the net loss of phosphate from the cell.

Intense exercise induces the degradation of adenine nucleotide and purine nucleotide synthesis via de novo pathway in the rat liver

Intense exercise induced the synthesis of purine nucleotide in the liver via a de novo pathway and these synthesized nucleotides were also degraded to nucleosides and excreted into urine.

Beta-adrenergic agonists stimulate the oxidative pentose phosphate pathway in the rat heart.

The results indicate that cardiac beta-adrenergic receptors and enzyme protein synthesis are involved in the stimulation of the cardiac oxidative pentose phosphate pathway.

Increased Adenine Nucleotide Degradation in Skeletal Muscle Atrophy

This characteristic decrease of AdNs suggests that increased nucleotide degradation contributes to the general pathophysiology of skeletal muscle atrophy.

Decreased resting levels of adenine nucleotides in human skeletal muscle after high-intensity training.

It is indicated that high-intensity intermittent exercise causes a decrease in resting levels of skeletal muscle adenine nucleotide without a concomitant indication of muscle damage.

Effects of ischemia on skeletal muscle energy metabolism in mice lacking creatine kinase monitored by in vivo 31P nuclear magnetic resonance spectroscopy

In vivo experimental evidence for the proposed biological significance of the creatine kinase (CK)/phosphocreatine (PCr) system in the energy metabolism of skeletal muscle revealed that the system plays a role in maintaining pH levels; the CK−/− mice showed a faster and more pronounced acidification than muscles of wild type and single knockout mutants.

Improving the preservation of isolated rat skeletal muscles stored for 16 hours at 4 degrees C.

The results showed that the addition of BDM and antioxidants to the bathing solutions improved the preservation of the function, metabolism, and cytoarchitecture of isolated skeletal muscles after cold storage for 16 hr.



Purine biosynthesis de novo in rat skeletal muscle.

Evidence is presented which suggests that the source of glycine and serine for purine biosynthesis is extracellular rather than intracellular, and the relative incorporation rates of the three precursors were formate greater than glycine greater than serine.

Purine metabolism in rat skeletal muscle.

Rat skeletal muscle can synthesize purine nucleotides de novo via "salvage" pathways from purine bases and nucleosides and under the conditions employed incorporation rates were adenosine adenine inosine/hypoxanthine guanosine guanine.

Effects of Ischemia on Tissue Metabolites in Red (Slow) and White (Fast) Skeletal Muscle of the Chicken

Results support the hypothesis that adenosine may be a mediator of skeletal muscle reactive hyperemia folio-wing short periods of ischemia and help clarify the role of vasodilator metabolites in ischemic skeletal muscle injury.

Relationship between Protein Synthesis and RNA Content in Skeletal Muscle

The extent to which alterations in rates ofprotein synthesis result from changes in the capacity for and the efficiency of protein synthesis in muscle is investigated.

Adenosine and active hyperemia in dog skeletal muscle.

The data indicate that the adenosine content of skeletal muscle is increased by contraction, and support the concept that adenosines may be a mediator of sustained active hyperemia.

Purine transport and metabolism in man: the effect of exercise on concentrations of purine bases, nucleosides and nucleotides in plasma, urine, leucocytes and erythrocytes.

Running, swimming and games were associated with a rise in hypoxanthine and xanthine excretion; exercise was probably the cause of the higher excretion during the day than at night, although turnover must rise.

Muscle fiber type composition of the rat hindlimb.

Fiber-type mass data are compared with physiological and biochemical data for the muscles taken from previous studies, and it is demonstrated that these functional properties are closely related to the proportions of muscle mass composed of the various fiber types.