De novo reverse transcription of HTLV-1 following cell-to-cell transmission of infection.

@article{Benovic1998DeNR,
  title={De novo reverse transcription of HTLV-1 following cell-to-cell transmission of infection.},
  author={S Benovic and T-W. Kok and Anne Stephenson and Joseph McInnes and Christopher Burrell and Peng Fei Li},
  journal={Virology},
  year={1998},
  volume={244 2},
  pages={294-301}
}
Analogous to transmission of human T-cell leukemia virus type 1 (HTLV-1) in vivo, an in vitro cell-to-cell infection model was established by coculturing MT-2 cells as virus donors and HUT78 cells as recipients. At a donor:recipient ratio of 1:2, cell fusion occurred and a new round of HTLV-1 genome replication was initiated in the cocultured cells. Newly synthesized unintegrated viral DNA was detected by Southern blot within 4-8 h and then increased between 8 and 48 h following cell mixing… CONTINUE READING