De novo protein sequencing by combining top-down and bottom-up tandem mass spectra.

@article{Liu2014DeNP,
  title={De novo protein sequencing by combining top-down and bottom-up tandem mass spectra.},
  author={Xiaowen Liu and Lennard J. Dekker and Si Wu and Martijn M. VanDuijn and Theo M. Luider and Nikola Toli{\'c} and Qiang Kou and Mikhail Dvorkin and Sonya Alexandrova and Kira Vyatkina and Ljiljana Pasa-Tolic and Pavel A. Pevzner},
  journal={Journal of proteome research},
  year={2014},
  volume={13 7},
  pages={3241-8}
}
There are two approaches for de novo protein sequencing: Edman degradation and mass spectrometry (MS). Existing MS-based methods characterize a novel protein by assembling tandem mass spectra of overlapping peptides generated from multiple proteolytic digestions of the protein. Because each tandem mass spectrum covers only a short peptide of the target protein, the key to high coverage protein sequencing is to find spectral pairs from overlapping peptides in order to assemble tandem mass… CONTINUE READING

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